KMID : 1123920210350020056
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Korean Journal of Oriental Physiology and Pathology 2021 Volume.35 No. 2 p.56 ~ p.63
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Study of Anti-inflammatory Activity of 7 Herbal Prescription Effective for Sleep Disorders and Atopic Dermatitis
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Choi Min-Jijn
Nguyen Ly Thi Huong Jeong Jeong-Hwa Park Min-Young Yoon Joo-Hee Lee Byung-Wook Yang In-Jun Shin Heung-Mook
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Abstract
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This study was conducted to find a candidate prescription with anti-inflammatory efficacy of 7 herbal prescriptions known to be effective in atopic dermatitis and sleep disorders in Korean medicine. The anti-inflammatory of the 7 herbal prescriptions extracts were evaluated by ELISA assay and Western blot assay. 7 herbal prescriptions, Seongyutang(SU), Danseontoetang(DST), Sotosajahwan(ST), Jisiljagyagsan(JJ), Seokchangpo(SCP), Wiryeongseon(WLS), Gogojohwan(GGJ) 30 % EtoH extract was used for in vitro experiments. In TNF-¥á + IFN-¥ã(TI) stimulated HaCaT cells, all 7 herbal prescriptions reduced TARC and MDC production, and JJ strongly reduced TARC and MDC production at 100 §¶/ml concentration. SCP strongly reduced MDC production at 10 §¶/ml and 100 §¶/ml concentration. In addition, in substance P(SP)/CRH stimulated HMC-1 cells, JJ strongly inhibited VEGF production at both 10 and 100 §¶/ml concentrations. In LPS/CRH stimulated Raw264.7 cells, all 7 herbal prescriptions significantly inhibited TNF-¥á. PMA + Ionomycin(PI)/CRH stimulated EL4 cells, SU significantly reduced IL-4 production at both concentrations of 10 and 100 §¶/ml. WLS significantly reduced IL-17 production at both concentrations of 10 and 100§¶/ml. This suggests that 7 herbal prescriptions have anti-inflammatory effects by reducing inflammatory cytokines in keratinocytes, mast cells and macrophages caused by inflammation. Therefore, it is expected that 7 herbal prescriptions that are effective for sleep disorders and atopic dermatitis can be used as therapeutic materials for sleep disorders and diseases associated with atopic dermatitis.
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KEYWORD
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Atopic dermatitis, Sleep disturbance, Herbal prescriptions, Comorbidity, Stress
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